羊骨髓肽及其钙螯合物对成骨细胞增殖和活性的影响

doi:10.11843/j.issn.0366-6964.2018.12.024

Abstract

Abstract:

Osteoblasts play bone-forming roles in the process of bone metabolism. Calcium-chelated bone marrow peptides (MP-Ca) was prepared and its effects on the proliferation and osteogenic activity of osteobalst (OB) were investigated and compared with calcium-free peptides of sheep bone marrow (MP). Prepared MP-Ca was identified via Fourier transform infrared spectrometer. The osteoblasts isolated from the skull of newborn SD rats were identified through alkaline phosphatase (ALP) and alizarin red staining. Different concentrations of test substance, including the positive control 17 β-estradiol, were added to the medium and the CCK-8 method was used to determine the optimal concentration for the subsequent study. The proliferation and the osteogenic activity of OB were expressed as OD_{450 nm} and the content of ALP and osteocalcin (BGP) in cell lysate prepared from MP-Ca, MP, estrogen treated OB cells at optimal concentrations. MP and MP-Ca showed no completely overlapped infrared spectra and apparent shifts of absorption peaks were observed, which indicated that MP-Ca was different form MP and it was prepared successfully. After ALP staining the isolated and purified OB cells appeared positive reaction. The results showed that OD_{450 nm}, ALP and BGP levels of the MP-Ca treated cells were significantly higher than those of the MP treatment (P<0.05 or P<0.01), but they were significantly lower than those of 17β-estradiol treated group (P<0.05 or P<0.01). It can be concluded that although inferior to 17β-estradiol, MP and MP-Ca were effective in promoting the proliferation of osteoblasts in vitro and enhance their bone formation activity, and MP-Ca was superior to MP.

CLC Number:

S857.161

JIN Shu-xiu, WANG Yu-hui, HUO Nai-rui, ZHENG Ming-xue, HAN Ke-guang, GU Shao-peng, TIAN Wen-xia, ZHANG Ding. Function of Bone Marrow Peptide and Its Calcium-Chelated Form in the Proliferation and the Osteogenic Activity of Cultured Osteoblasts[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2745-2752.

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Function of Bone Marrow Peptide and Its Calcium-Chelated Form in the Proliferation and the Osteogenic Activity of Cultured Osteoblasts

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